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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: Palmitoylation of Progressive Rod-Cone Degeneration (PRCD) Regulates Protein Stability and Localization
doi: 10.1074/jbc.M116.742767
Figure Lengend Snippet: The gBlocks fragments of human PRCD wild type and mutant gene sequences were synthesized from Integrated DNA Technologies used for cloning into pGAG-EGFP vector for cell culture and subretinal injection studies The restriction sites for cloning in to pGAG-EGFP vector are underlined, and the bolded sequences in the 3′ sites represent the HA epitope tag. The changes made in the sequence according to human mutations are underlined and bolded.
Article Snippet: The human PRCD constructs encoding wild type and mutants (C2Y and V30M) with the C-terminal HA epitope-tagged
Techniques: Mutagenesis, Synthesized, Clone Assay, Plasmid Preparation, Cell Culture, Injection, Sequencing